ABSTRACT
A 28-year-old male patient with coughing was treated with intramuscular injection of lincomycin,oral Kesouting granules and erythromycin in a local clinic.One day later,erythema occurred on the head and face,and rapidly spread to the trunk and limbs.Three days later,a large number of densely distributed needle tip-to millet-sized pustules occurred on the generalized erythema all over the body with fever and burning pain sensation of the skin.Skin examination revealed diffuse edematous erythema with obvious hyperemia on more than 90％ of the body,a large number of needle tip-to millet-sized whiteyellowish pustules on the erythema on the hair line,face,flexor aspect of the extremities and flexural site of the trunk (axillary and inguinal regions),and bilateral lower-extremity swelling.No erythema or erosion was observed on the oral mucosa or genital mucosa.Histopathological examination of skin lesions on the right elbow showed local intraepidermal and subcorneal pustules,necrotic keratinocytes and neutrophil aggregation in the pustules,and infiltration of lymphocytes and eosinophils in the superficial dermis.Laboratory examination showed elevated white blood cell counts (26.9 × 109/L),neutrophils proportion (Segment,0.88),C-reactive protein level (127.89 rmg/L),aspartate aminotransferase level (44.2 U/L) and alanine transarninase level (77.3 U/L),but decreased proportion of lymphocytes (0.08).The patient was diagnosed with acute generalized exanthematous pustulosis.After admission,the patient was treated with intravenous drips of methylprednisolone at a dose of 60 mg/d.Then,the condition was rapidly relieved,and the dosage was rapidly reduced.The patient was discharged on day 7.
ABSTRACT
Objective To explore the changes and clinical significance of regulatory T cell/T help cell 17 (Th17) in the peripheral blood from patients with dermatomyositis (DM).Methods Thirty-six patients with DM were recruited and thirty-six healthy individuals were enrolled as normal controls.The percentage of regulatory T cell/Th17 cell was assessed by flow cytometry and the mRNA levels of Foxp3/RORγt were detected by real-time quantitative reverse wanscription polymerase chain reaction (RT-PCR).The plasma levels of TGF-β1/IL-17 were measured by enzyme linked immunosorbent assay (ELISA).T-test was used for statistical analysis and Pearson's linear analysis were used for correlation analysis.Results The percentage of CD4+ Foxp3+ T cell,the mRNA expression of Foxp3 and the plasma levels of TGF-β1 decreased significantly in patients with DM than in normal controls [(7.2±2.3)％ vs (9.1±3.2)％,(0.44±0.19) vs (0.62±0.25),(37 400±4814) pg/ml vs (41 200±6348) pg/ml,P＜0.05].The percentage of Th17 cell,the mRNA expression of RORγt and the plasma levels of IL-17 increased in patients with DM compared with normal controls [(1.22±0.41)％ vs (0.83±0.14)％,(0.85±0.34) vs (0.61±0.21),(77±32) pg/ml vs (58±20) pg/ml,P＜0.05].There was negative correlation between the percentage of regulatory T cell,the mRNA levels of Foxp3,the plasma levels of TGF-β1 and the serum levels of CK in patients with DM (r=-0.573,-0.593,-0.618,P＜0.05).There was positive correhtion between the percentage of Thl7 cell,the mRNA levels of RORγt,the plasma levels of IL-17 and the serum levels of CK in patients with DM (r=0.582,0.609,0.623,P＜0.05).Conclusion The imbalance of regulatory T cell/Th17 cell may exist in the peripheral blood of patients with DM.Regulatory T cell/Th17 cell may be associated with the pathology of DM.
ABSTRACT
Objective By exploring the effects of recombinant human bone morphogenetic protein (rhBMP)2 and rhBMP13 on chondrocytes proteoglycan production and phenotype expression to establish the theoritical mechanisms for the treatment of disc degeneration with chondrocytes transplantation plus BMPs.Methods The dose-dependent effects of rhBMP2 and rhBMP13 on PG protein synthesis and gene expression were detected under different concentrations (0,25,125,and 625 ng/ml).The sulfated-glycosaminoglycan (s-GAG) in the culture media and the pericellular matrix was measured with a 1,9-dimethyl-methylene blue (DMMB) colorimetric assay.Reverse transcriptase polymerase chain reaction (RT-PCR) was performed respectively to quantify the relative abundance of aggrecan Mrna.Cell proliferation was examined by Hoechst Dye assay.Results All rhBMP2 and rhBMP13 in different concentrations could significantly increase s-GAG synthesis and gene expression in chondrocytes (P＜0.05).And at the same concentration.rhBMP2 was more potent than rhBMP13 on s-GAG synthesis.Hoechst Dye assay showed neither rhBMP2 nor rhBMP13 had significant effect on cell proliferation.Conclusion rhBMP2 and rhBMP13 are able to upregulate s-GAG synthesis,in addition,rhBMP2 is more potent than rhBMP13 on aggrecan gene expression regulation,but rhBMP2 and rhBMP13 do not have significant effect on chondrocyte proliferation.